Lone Wolf and Cub: Baby Cart at the River Styx

Trailed by a clan of female ninja, Ogami is paid to assassinate a clan traitor accompanied by three killers known as the Gods of Death.
In the second film of the Lone Wolf and Cub series, Ogami Itto battles a group of female ninja in the employ of the Yagyu clan and must assassinate a traitor who plans to sell his clan's secrets to the Shogunate.